Journal: NPJ Regenerative Medicine

Article Title: CD73 overexpression in ADSCs accelerates bladder repair by regulating the NFκB/NLRP3/caspase-1 signaling axis in neurogenic bladder rats

doi: 10.1038/s41536-026-00454-1

Figure Lengend Snippet: A Immunofluorescence staining results of ADSCs in the bladder tissues of each group of rats. Red represents ADSCs, green represents βIII-tubulin, and blue represents DAPI. Scale bar = 20 µm. B Immunohistochemical results of CXCR4 in the bladder tissues of each group. The green arrows indicate regions of high CXCR4 expression. Scale bar = 50 µm. C Immunofluorescence staining results of CD73 and SDF-1 in the bladder tissues of each group. Red represents SDF-1, green represents CD73, and blue represents DAPI. Scale bar = 20 µm. D Quantitative results of ADSCs in the bladder tissues of each group ( n = 5). E Quantitative results of SDF-1 expression in the bladder tissues of each group ( n = 3). F In vitro, western blot results of SDF-1 in each group under conditioned medium. G Quantitative results of SDF-1 expression of each group ( n = 3). H Cell migration assay results of CD73⁺ ADSCs after SDF-1 gene knockdown. I Quantitative results of cell migration assays for ADSCs in each group ( n = 3). Data presented as ±SD. * indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001. Full-length blots/gels are presented in Supplementary Fig. . Full-section IHC are presented in Supplementary Fig. .

Article Snippet: The following primary antibodies were used: VEGF (diluted 1:200, Servicebio, Wuhan, Hubei, China), CD73 (diluted 1:200,Servicebio, Wuhan, Hubei, China), DAPI (diluted 1:200, Servicebio, Wuhan, Hubei, China), βIII-tublin (diluted 1:200, Servicebio, Wuhan, Hubei, China), SDF-1(diluted 1:200, Servicebio, Wuhan, Hubei, China), Caspase-1(diluted 1:200, Servicebio, Wuhan, Hubei, China).

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Expressing, In Vitro, Western Blot, Cell Migration Assay, Knockdown, Migration

Journal: NPJ Regenerative Medicine

Article Title: CD73 overexpression in ADSCs accelerates bladder repair by regulating the NFκB/NLRP3/caspase-1 signaling axis in neurogenic bladder rats

doi: 10.1038/s41536-026-00454-1

Figure Lengend Snippet: A Western blot results of AKT, p-AKT, NFκB, and p-NFκB in each group, in vitro. B Western blot results of caspase-1 and NLRP3 in each group, in vitro. C Quantitative analysis of p-AKT and p-NFκB expression ( n = 3). D Quantitative results of p-mTOR expression in the bladder tissues of each group ( n = 3). E Immunofluorescence staining results of p-mTOR in the bladder tissues of each group. Red represents p-mTOR, green represents βIII-tubulin, and blue represents DAPI. Scale bar = 20 µm. F Quantitative analysis results of caspase-1 and NLRP3 expression ( n = 3). G HE staining results of bladder tissues from each group. Scale bar = 100 µm. H Immunohistochemical results of PI3K in the bladder tissues of each group. Scale bar = 50 µm. I Immunohistochemical results of HIF-1α in the bladder tissues of each group. Scale bar = 50 µm. J – L Western blot results of p-PI3K, p-AKT, and p-mTOR were inhibited individually in each group in vitro. M Western blot results HIF-1α in ADSCs in vitro. Data presented as ±SD. * and # indicates P < 0.05, ** and ## indicates P < 0.01, and *** and ### indicates P < 0.001. Full-length blots/gels are presented in Supplementary Fig. . Full-section IHC are presented in Supplementary Fig. .

Article Snippet: The following primary antibodies were used: VEGF (diluted 1:200, Servicebio, Wuhan, Hubei, China), CD73 (diluted 1:200,Servicebio, Wuhan, Hubei, China), DAPI (diluted 1:200, Servicebio, Wuhan, Hubei, China), βIII-tublin (diluted 1:200, Servicebio, Wuhan, Hubei, China), SDF-1(diluted 1:200, Servicebio, Wuhan, Hubei, China), Caspase-1(diluted 1:200, Servicebio, Wuhan, Hubei, China).

Techniques: Western Blot, In Vitro, Expressing, Immunofluorescence, Staining, Immunohistochemical staining